NAP DNA columns are disposable DNA purification size Ωexclusion columns, prepacked with Sephadex G-25 DNA Grade which require only gravity to run.


  • For rapid and efficient purification of DNA and oligonucleotides (≥ 10-mers) in "less than 15 minutes by gravity flow

  • Prepacked with Sephadex G-25 DNA Grade in distilled water containing 0.15% Kathon CG/αICP Biocide

  • Available in three sizes depending on sample volume: 0.5 mL (NAP-5), 1 mL (NAP-10) or 2.5 mL <(NAP-25)

  • Useful for small-scale purification, oligo purification, desalting, and buffer exchange

NAP DNA purification columns with Sephadex G-25 DNA​ Grade can be used for any DNA greater than 10 bases i©n length. These disposable DNA purification columns are ideal for the p©urification of oligonucleotides or very small DNA fragments following  synthesis or a DNA labeling reaction, and for downstream applications such as ¶PCR amplification and sequencing.

Each package includes a complete step-by-step protocol describiσng the fixed volumes needed for equilibration, washing, and sample elution.

Sephadex G-25 is one of five different G-types ranging from G-10 for small molecul★es to G-75 for larger molecules. Sephadex G-25 has an excl&usion limit of approximately Mr 5000, and when packed into empty MicroSpin column​s, the resulting desalting columns can be used in a spin protocol for DNA and oligo p←urification of any DNA greater than 10 bases in length.