TaqMan® Fast Advanced Master Mix has been designed to match or e®xceed the performance of standard master mixes, delivering shorter run times (<§40 minutes) with results equal to or better than what is achieved today. TaqMan&® Fast Advanced Master Mix contains AmpliTaq® Fast DNA Polymera↔se, uracil-N glycosylase (UNG), dNTPs with dUTP, ROX™ d≥ye (passive reference), and optimized buffer components. It is supplied at a 2X∏ concentration.

Features of the TaqMan® Fast Advanced Master Mix include:

• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and sβpecificity compared to standard mixes in standard mo∞de
• Engineered for enhanced benchtop stability—stable at room temperature for up t​o 72 hours in preassembled reactions
• Optimized for multiplexing—validated for duplexing with exogenous' and endogenous internal positive control assays
• Reduced run times on fast and standard instrumentation—optimized for fast mode on fast in≥struments and fast cycling conditions on standard instruments
• Seamlessly transitions into your experiments—validated with Applied Biosystems® TaqMan® As•says for Gene Expression and microRNAs, and TaqMan® Arra↓y Micro Fluidic Cards

Best-in-Class Performance
TaqMan® Fast Advanced Master Mix has been designed to provide performance equal to or bet£ter than the results you currently expect from your standa®rd master mix. The master mix has been benchmarked against the leading supplie£rs' standard and fast master mixes to help ensure that ₹it succeeds in providing best-in-class sensitivity, accuracy,​ dynamic range, and specificity. The impressive sensitivity of ×the mix showcased in Figure 1 compares the C_tvalues of TaqMan® Fast Advanced Master Mix and TaqMan® Universal PCR Master Mix across a p→anel of gene expression assays.

Benchtop Stability for High-Throughput Handling and Convenience
TaqMan® Fast Advanced Master Mix has been engineered to retain a high leve>l of performance in preassembled reactions for up to 72 hours. The stabi≥lity of this mix provides users of high-throughput, liquid handling systems the assuran∑ce that the results on the first plate will mimic those of the γlast plate. Figure 2 shows an assay that was run upon assembly (time 0) a≥nd after 72 hours of incubation at 30°C, simulating the most extreme room temperature scena☆rio.

For those with less extreme throughput needs, the enhanced stability of this master mix provides÷ an overall added convenience to your workflow, as you are no longer constrained to immediately λrunning your plates upon assembly.

Optimized for Multiplexing
At Life Technologies, we realize that confidence is paramount when it comes to your results. For ♥added confidence in the results you see in every well, ±TaqMan® Fast Advanced Master Mix has been designed to help deliver accurate results for duple≥x reactions using an internal positive control (IPC). πFigure 3 shows results for the experimental target β-actin (gene name ≈ACTB), which was serially diluted and amplified in single-target reactions and duplex reac☆tions.

Validated for MicroRNA Assays
TaqMan® Fast Advanced Master Mix has been validated for use with multiple real-time PCR ¶applications, including microRNA assays. The formulati™on provides high specificity and dynamic range, the two most c→ritical performance attributes that define successful results when working with φmicroRNAs. The data in Figure 4 demonstrate excellent PCR linearity over a 6-log range of ✔input template.

Reduced Run Times on Standard Instrumentation
TaqMan® Fast Advanced Master Mix has been optimized for use withΩ both fast and standard instrumentation, enabling researchers who ♦currently own standard instruments to also reap the performance benefi♣ts and time savings this mix provides. Figure 5 showcases the impressive resul'ts achieved when using TaqMan® Fast Advanced Master Mix under fast therm₹al cycling conditions on Applied Biosystems® 7300 Real-Time PCR Syst→em. The mix has been tested with all Applied Biosystems standard real-time PCR& instrumentation (7900HT, 7500 and 7300 systems) to enable success independent of wδhether or not you own a fast-enabled instrument.

詳細說(shuō)明(míng)

常用(yòng)規格

內(nèi)容和(hé)儲存

Contains 1 tube (1 ml) 2X TaqMan® Fast Advanced Master Mix s∞ufficient for 100 reactions

TaqMan® Fast Advanced Master Mix contains AmpliTaq® Fast DNA Polymerase, Uracil-N₽ glycosylase (UNG), dNTPs (with dUTP), ROX™ dye (passive reference), and optimized buffer.

Upon receipt, store at -20°C. After opening, store at 4°C¥.